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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
15/10/2020 |
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Data da última atualização: |
15/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
REZENDE, L. C. de; CARVALHO, A. L. de A.; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; SILVA, L. G.; PINTO. Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V. de; MASCARIN, G. M.; BETTIOL, W. |
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Afiliação: |
LARISSA CASTRO DE REZANDE, UFLA; ANDRÉ LUIZ DE ANDRADE CARVALHO, UFLA; LÚCIO BERTOLDO COSTA, FCA-UNESP; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; LUCAS GUEDES SILVA, FCA-UNESP; ZAYAME VEGETTE PINTO, FCA-UNESP; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; FLÁVIO HENRIQUE VASCONCELOS DE MEDEIROS, UFLA; GABRIEL MOURA MASCARIN, CNPMA; WAGNER BETTIOL, CNPMA. |
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Título: |
Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
World Journal of Microbiology & Biotechnology, v. 36, n. 8, article 113, 2020. |
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ISSN: |
1573-0972 |
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DOI: |
https://doi.org/10.1007/s11274-020-02882-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these optimal conditions, T. erinaceum T-12 and T-18, and T. harzianum T-15 were also cultivated, but only LQC-96 efficiently parasitized S. sclerotiorum, precluding sclerotium myceliogenic germination. Our findings propose optimal fermentation conditions that maximize conidial production of T. asperelloides as a potential biofungicide against S. sclerotiorum. MenosAbstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these opt... Mostrar Tudo |
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Palavras-Chave: |
Shite mold; Submerged spores. |
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Thesagro: |
Fermentação; Fungo Para Controle Biológico; Trichoderma. |
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Thesaurus Nal: |
Biological control; Biological control agents; Bioreactors; Experimental design; Mass rearing; Molds (fungi); Solid state fermentation. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03117naa a2200397 a 4500
001 2125536
005 2020-10-15
008 2020 bl uuuu u00u1 u #d
022 $a1573-0972
024 7 $ahttps://doi.org/10.1007/s11274-020-02882-7$2DOI
100 1 $aREZENDE, L. C. de
245 $aOptimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum.$h[electronic resource]
260 $c2020
520 $aAbstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these optimal conditions, T. erinaceum T-12 and T-18, and T. harzianum T-15 were also cultivated, but only LQC-96 efficiently parasitized S. sclerotiorum, precluding sclerotium myceliogenic germination. Our findings propose optimal fermentation conditions that maximize conidial production of T. asperelloides as a potential biofungicide against S. sclerotiorum.
650 $aBiological control
650 $aBiological control agents
650 $aBioreactors
650 $aExperimental design
650 $aMass rearing
650 $aMolds (fungi)
650 $aSolid state fermentation
650 $aFermentação
650 $aFungo Para Controle Biológico
650 $aTrichoderma
653 $aShite mold
653 $aSubmerged spores
700 1 $aCARVALHO, A. L. de A.
700 1 $aCOSTA, L. B.
700 1 $aHALFELD-VIEIRA, B. de A.
700 1 $aSILVA, L. G.
700 1 $aPINTO. Z. V.
700 1 $aMORANDI, M. A. B.
700 1 $aMEDEIROS, F. H. V. de
700 1 $aMASCARIN, G. M.
700 1 $aBETTIOL, W.
773 $tWorld Journal of Microbiology & Biotechnology$gv. 36, n. 8, article 113, 2020.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
06/05/2010 |
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Data da última atualização: |
20/05/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PARENTONI, S. N.; SOUZA JUNIOR, C. L.; ALVES, V. M. C.; COELHO, A. M.; GUIMARAES, P. E. D. O.; PACHECO, C. A. P.; OLIVEIRA, A. C. D.; GUIMARAES, C. T.; MAGALHAES, J. V. D.; VASCONCELOS, M. J. V. D.; MEIRELLES, W. F.; GUIMARAES, L. J. M.; SILVA, A. R. D.; SCHAFFERT, R. E. |
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Afiliação: |
SIDNEY NETTO PARENTONI, CNPMS; CLÁUDIO L. SOUZA JÚNIOR, ESALQ-USP; VERA MARIA CARVALHO ALVES, CNPMS; ANTONIO MARCOS COELHO, CNPMS; PAULO EVARISTO DE O GUIMARAES, CNPMS; CLESO ANTONIO PATTO PACHECO, CPATC; ANTONIO CARLOS DE OLIVEIRA, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; MARIA JOSE VILACA DE VASCONCELOS, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; LAURO JOSE MOREIRA GUIMARAES, CNPMS; ADELMO RESENDE DA SILVA, CNPMS; ROBERT EUGENE SCHAFFERT, CNPMS. |
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Título: |
Genetics of phosphorus efficiency in tropical maize. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON PLANT-SOIL INTERACTIONS AT LOW pH, 7., 2009, Guangzhou. Plant-Soil Interactions at Low pH: nutriomic approach. Proceedings... Guangzhou: South China University of Technology, 2009. |
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Páginas: |
p. 134. |
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Idioma: |
Português |
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Palavras-Chave: |
Maize. |
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Thesagro: |
Milho. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00991naa a2200301 a 4500
001 1749079
005 2021-05-20
008 2009 bl --- 0-- u #d
100 1 $aPARENTONI, S. N.
245 $aGenetics of phosphorus efficiency in tropical maize.
260 $c2009
300 $ap. 134.
650 $aMilho
653 $aMaize
700 1 $aSOUZA JUNIOR, C. L.
700 1 $aALVES, V. M. C.
700 1 $aCOELHO, A. M.
700 1 $aGUIMARAES, P. E. D. O.
700 1 $aPACHECO, C. A. P.
700 1 $aOLIVEIRA, A. C. D.
700 1 $aGUIMARAES, C. T.
700 1 $aMAGALHAES, J. V. D.
700 1 $aVASCONCELOS, M. J. V. D.
700 1 $aMEIRELLES, W. F.
700 1 $aGUIMARAES, L. J. M.
700 1 $aSILVA, A. R. D.
700 1 $aSCHAFFERT, R. E.
773 $tIn: INTERNATIONAL SYMPOSIUM ON PLANT-SOIL INTERACTIONS AT LOW pH, 7., 2009, Guangzhou. Plant-Soil Interactions at Low pH: nutriomic approach. Proceedings... Guangzhou: South China University of Technology, 2009.
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